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Microbiology

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Microbiology

Overview

Department of Microbiology performs academic and diagnostic services. The subject is taught to 2nd MBBS students as per CBME guidelines. Post graduation (MD) was started in 2020 and there are 12 students perusing MD in the department.

Department of Microbiology is an NABL accredited laboratory for all the tests in  scope of Serology, Microbiology &molecular testing. This was the first government laboratory to achieve NABL accreditation in molecular testing in Gujarat. It serves as DPHL (district public Health lab) for Ahmedabad rural area that caters to outbreak investigations and environmental surveillance for the villages falling under Ahmedabad rural.

 It’s a Model diagnostic laboratory for Gujarat for HBV & HCV viral load testing under NVHCP (National Viral Hepatitis Control Program) program.

Department was first to initiate HIV 1 Viral load testing for HIV positive patients under NACP. This lab is one of the four labs operational in Gujarat & caters 13 ART centres from North & Central Gujarat.

We are also one of the 29surveillance lab across India for surveillance for viral Hepatitis under NPSVH( National Program For Surveillance of Viral Hepatitis) as part of MOU with NCDC.

Department serves as a training centre for NVHCP and HIV-1 viral load testing for state of Gujarat.

Along with BSL 2 labs, department is also equipped with BSL 3 laboratory.

It’s a key department for infection control & prevention activities in the hospital. It carries out various trainings for hospital staff, AMR data analysis and dissemination to clinicians, environmental sampling, audits and participations in policy formulations for infection prevention in the hospital.

We are a participant lab for AMR surveillance program under GUJSAR run by CDC.
Facilities
Department Of Microbiology - Diagnostic & Teaching facility

Sr No

Name of the test

Test Principal

Bacteriology

1

Aerobic culture and Sensitivity testing     

 ( Biological samples)

Conventional culture and AST

2

Diphtheria Culture

 

3

Anaerobic culture

Conventional

4

Automated Blood Culture

BD Bactec system for Automated Bi culture

5

Gram Staining

 

6

ZN staining

 

7

Albert Staining

 

8

Giemsa staining

 

9

ZN stain for Lepra bacilli

 

10

Environmetal surveillance

Air, Water and Surface Samples culture

Serology

11

HbsAg detection

ELISA

12

HbsAg detection

Immunochromatography

13

ASO

Latex agglutination

14

RA factor

Latex agglutination

15

CRP

Latex agglutination

16

WIDAL slide and tube

Latex agglutination

17

RPR

Passive agglutination

18

TPHA for Syphilis

Passive agglutination

 

 

 

Virology

19

NS1 ag detection for Dengue

ELISA

20

IgM anti Dengue Ab

ELISA

21

IgM anti CHIK Ab

ELISA

22

IgM anti HAV Ab

ELISA

23

IgM anti HEV Ab

ELISA

24

Anti HCV Ab

ELISA

25

Anti HCV Ab

Immunochromatography

26

IgM anti TOXOPLASMA Ab

ELISA

27

IgG anti TOXOPLASMA Ab

ELISA

28

IgM anti Rubella Ab

ELISA

29

IgG anti Rubella  Ab

ELISA

30

IgM anti Cytomegalo  Ab

ELISA

31

IgG anti Cytomegaly Ab

ELISA

32

IgM anti Herpes Ab

ELISA

33

IgG anti Herpes Ab

ELISA

HCTS centre & Immunology

34

Anti HIV antibody

ELISA

35

Anti HIV antibody

Rapid

36

DBS

DNA PCR

Mycology

37

Microscopy for Fungal identification

 

38

KOH

 

39

India ink

 

40

Fungal Culture

 

 

41

Stool microscopy

Wet mount

42

Modified ZN stain for parasites

 

Molecular

43

Influenza

RT PCR

44

COVID 19

RT PCR

45

Hepatitis B viral load

DNA PCR

46

Hepatitis C viral load

RT PCR

47

Dengue serotyping

RT PCR

NPSVH (national program for surveillance of viral Hepatitis)

48

IgM anti HAV

CLIA

49

IgM Anti HBc

CLIA

50

Anti HCV antibody

CLIA

51

HBsAg

CLIA

NACP (National AIDS Control Program)

52

HIV 1 viral load

RT PCR

Outbreak investigation

AMR surveillance













Department Of Microbiology - Diagnostic & Teaching facility

TESTS DONE IN VARIOUS LABS

Serology –

ELISA for Hepatitis B surfaceantigen , Measles IgM antibody, Brucella IgM antibody

Rapid tests – hepatitis B surfaceantigen , RA factor for Rheumatoid arthritis, CRP, ASO test

for rheumatic fever , Widal test  for Typhoid, RPR & TPHA test  for syphilis

Virology –

ELISA for Dengue NS1 Ag, DengueIgM antibody, hepatitis C, Hepatitis A, Hepatitis E,

Chikungunya IgM antibody,Toxoplasma IgM & IgG antibody, Herpes IgM & IgG antibody,

Rubella IgM & IgG antibody,Cytomegalo  IgM & IgG antibody

Rapid test- Hepatitis C antibody

Immunology – HIV antibody ELISA & rapid, DBS collectionfor HIV DNA PCR

Bacteriology –

Microscopy

wet mount,stain- Gram, ZN for Mycobacterium tuberculosis, Albert stain for

Diphtheria ,Giemsa, etc

Culture & antibioticsusceptibility testing for diagnosis of infectious diseases like Cholera,

Typhoid, meningitis, TB, UTI,Pneumonia, Septicaemia, Diphtheria , dysentery, pharyngitis   

etc (from  Pus, Blood, Urine, CSF, Sputum, Stool, fluids,Tissue etc)

Parasitology –

Diagnosis of parasitic diseases likeAmoebiasis, intestinal worms, Filariasis etc by

 Stool routine Micro

ModifiedZN  stain for stool parasites

Mycology –

Diagnosis of Fungal infections ofskin, hair, nail ,ear, eyes etc by

                                Microscopy(KOH) & culture

Molecular –

Diagnosis of various viralinfections like COVID 19, Influenza, Hepatitis B & C viral load, HIV 1

Viral load, Dengue Serotyping etcby RT PCR

Infection control –

surveillance of Air, Water &surface in hospital for infection control & prevention

AMR surveillance

National viral hepatitis control program(NVHCP) forHepatitis B & Hepatitis C

National program for surveillance of Viral Hepatitis (NPSVH)

National AIDS control program – Viral load testing for HIV 1by RT PCR

District Public health lab (DPHL) – Samples received fromAhmedabad rural & Surendra Nagar

Outbreak investigation

 

 


Under Graduate Teaching
  • Department of Microbiology is teaching 2nd year MBBS students as per CBME curriculum.
  • It has Lecture Series, Small Group Discussion, SDL (self directed learning), Tutorials, U.G Practical (DOAP) & Demonstration Session as well as Seminars.  
  • Learning of AETCOM topics through lecture & practical performance under departmental supervision.
Infrastructure
Infrastructure & Equipments

 

THE FOLLOWING LABS AREOPERATIONAL IN THE DEPARTMENT  UNDER THECONTROL & SUPERVISION OF DEPARTMENT OF MICROBIOLOGY

 

Sr no

Name of the lab

Size in sq mt

a)                                                 

Bacteriology including anerobic culture

34.71

b)                                                

Media preparation and storage area

20.26 

           

c)                                                 

Autoclaving room

 

19.43

d)                                                

Washing and drying room:

20.54

 

e)

Serology

30.68

f)

Virology

30.68

g)

Parasitology

34.71

 

h)

Mycology

34.71

 

i)

TB lab

31.46

j)

Immunology

31.46

k)

BSL 2- Molecular lab for COVID 19& influenza

58.93

 

l)

BSL 2- Molecular lab for HBV,HCV viral load

82

m)

BSL 2- Molecular lab for HIV 1 viral load

56.4

n)

BSL -3 Molecular lab

50.53

 

Research lab

Equipments

Thermocycler Realtime-1

Thermocycler – Conventional-1

Gel Doc System-1

Refrigerated Microcentrifuge-1

Weighing Scale-1

Deep Freeze-1

Biosafety cabinet-2

58.94

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 OFFICE ACCOMODATION & OTHERINFRASTRUCTURE IN DEPARTMENT

Demonstration Room:

 

No

Size

Capacity

2

Demonstration room no. 1 -   60 sq. mt.

Audio-Visual equipment available: Yes (OHP projector and Slide projector)

75 students

Demonstration room no. 2 -   60 sq.mt.

Audio-Visual equipment available: Yes (OHP projector and Slide projector)

 

75 students

Practical laboratories

1

260.05 sq.mt.

preparation room

   Size -14.63 sq.mt.

   Sinks – 26

   Cupboard for storage -adequate

    Number of Microscopes: 60

    OHP available

 

75 students 

Departmental Library-cum-Seminar Room

1

30.07 sq. mt.

 

15 students

Professor and H.O.D. 

1

26.64 sq. mt.

 

Associate Professor/Reader :     

2

1.   16 .8 Sq mt

2.    17.9 Sq mt

 

Asst. Professor/Lecturers :  

2

16.0 Sq mt

12  Sq mt

 

Tutors/Demonstrators.

1

51.5 Sq mt

 

Non-teaching

1

26.1 sq. mt.

 

Clerical staff                        

1

13.21 Sq mt

 

Museum

1

125 sq mt

 

 

 

Equipment list

Equipments inDepartment of Microbiology

No

Name of the item

Quantity

1

Refrigerators double door

5

2

Autoclave (Vertical)

3

3

VDRL Rotator

2

4

Vortex Mixer

5

5

Binocular Microscope

5

6

Binocular Microscope with dark ground attachment

1

7

Binocular  Microscope-Inverted

1

8

Deep freeze (-20 degree Celsius)

4

9

Digital pH meter

1

10

Distilled water plant

1

11

B.O.D. incubator

2

12

Laminar Flow

2

13

Centrifuge, electrical high power

5

14

Over head projector

2

15

CO2 incubator

1

16

Bio Safety Cabinet Type 2A

5

17

Conventional Thermocycler

1

18

Gel Doc System

1

19

Real Time Thermocycler

4

20

 Automated  Microbial Growth Detection System ( Bactec)

1

21

 Millipore water purification system

1

22

Balance Digital

1

23

Microcentrifuge - 1.5 ml tubes ( 4 tubes)

1

24

Microcentrifuge - PCR tube Strip

1

25

ELISA Washer

1

26

ELISA reader

1

27

Hot Air Oven

1

28

Incubator

3

29

Digital Colony Counter

1

30

Water bath (Serological ) 56 degree Celsius

1

31

Electrical Loop Sterilizer

2

32

Automated Nucleic acid Extraction and Real time PCR machine complete set (Abbot)

1

33

Fogger machine

1

34

Automated RNA Extraction system ( QIA symphony SP system)

1

35

Table Top (low speed)  centrifuge

1

36

Deep Freeze- -80c

1

37

CLIA (Autoanalyzer Immunoassay)

1

Staff Information
Sr No Name Designation View
1 Dr Nidhi Sood Professor & Head View
2 Dr. Rosy Parmar Associate Professor View
3 Dr Bhaumik Patel Associate Professor View
4 Dr Palak Dipakkumar Rao Assistant Professor View
5 Dr. Dhara Modi Assistant Professor View
6 Dr. Asha Mandalia Assistant Professor View
7 Dr Jaimin Desai Tutor View
8 Dr Jolly Patel Junior Resident / Tutor View
9 Dr. Naushin moledina Junior Resident / Tutor View
10 Dr. Prit Moradiya Junior Resident View
11 Dr. Het Contractor Junior Resident View
12 Dr Dhruvi Patel Senior Resident View
13 Dr Devanshi S. Rangani Senior Resident View
14 Dr. Rutu Chapla Senior Resident View
15 DR Bhojani siddhi Vallabhbhai R3 View
16 DR Ravat Manashvi Lakhansinh R3 View
17 DR Palkhiwala Mansi R3 View
18 DR Mitsu Manharbhai Parsania R3 View
19 DR AASTHA SUDIP KINARIVALA R2 View
20 DR Sarvaiya Anjaniba R2 View
21 DR RACHIT SARDARSINH NINAMA R2 View
22 DR Mayuri Nareshbhai Panchal R1 View
23 DR DhruvibenPiyushkumar Patel R1 View
24 DR Bhadarka Krishna Vrajlal R1 View
25 DR Sejpal Dhvani Atulbhai R1 View
26 DR Jadav Yashkumar Sureshbhai R1 View
Staff Attendance
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Subject Curriculum
CBME based UG & PG Curriculum
Department of Microbiology is teaching 2nd year MBBS students as per CBME curriculum. 
Competencies to be acquired in Microbiology
Postgraduate teaching is also CBME based

https://www.nmc.org.in/wp-content/uploads/2022/11/MD-Microbiology-revisedGuidelines.pdf 
Monthly Teaching Schedule
Sr No Teaching Schedule Month Teaching Schedule Short Description Teaching Schedule Date Download
1 July 2024 08/07/2024 Download
Students Notes
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Results of Examination
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Role of Honour
Contents Not Found!
Publication
Sr No Publication Title Publication Short Desc Posted Date Download
1 Total list of publications of department in last three years Total list of publications of department in last three years 26/06/2024 Download
2 List of Books in departmental library List of Books in departmental library 26/06/2024 Download
3 List of Journals in departmental library List of Journals in departmental library 26/06/2024 Download
Achievements
Contents Not Found!
Events
Sr No Events Year Events List Download
1 2024

Any conference, workshop or CME organized by the departmentin last 3 years (after 1/1/2021), if so, provide the details

S No

Title

Conference/ workshop/ CME

Supporting agency, if any name of agency

No of participants

1

HBV & HCV Viral load Testing Under NVHCP

workshop

Government of Gujarat

41

 

 

 

 

 

 


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