COMPETENCY BASED CURRICULUM OF BIOCHEMISTRY

NO.

COMPETENCY
The student should be able to

Learning Objectives (At the end of session, Phase-1 MBBS student shall be able to)

No. of Hours Required

Teaching Learning method

Assessment Type

Topic: Basic Biochemistry

Number of procedures that require certification: (NIL) 

BI1.1

Describe the molecular and functional organization of a cell and its sub- cellular components.

BI1.1. A -  Cell Organization & Subcellular Organelles

1. Describe Cell Structure & Organization

1

Lecture 

LAQ - Short Notes, SAQ & Viva

2. Enumerate various Subcellular Organelles -  with its Structure 

Lecture 

3. Enumerate Functions of Subcellular Organelles & its Clinical Importance

Lecture

BI.1.1.B - Cell Membrane & Transport Mechanisms 

1. Describe Fluid Mosaic Model of Cell Membrane

1

Lecture

2. Draw Fluid Mosaic Model with proper labelling correctly

Lecture

3. Write down Classification of  Transport Mechanism - Active, Passive & Pump with their examples

Lecture

3. Describe Active Transport Mechanism with Correct Diagram & Clinical Importance

Lecture

3. Describe Passive Transport Mechanisms with Correct Diagram  &  its Clinical Importance

Lecture

4. Describe Pump Mediated Transport with correct Diagram & Clinical Importance

Lecture

Topic: Enzyme

BI2.1

Explain fundamental concepts of  enzyme, isoenzyme, alloenzyme, coenzyme & co-factors. Enumerate  the main classes of IUBMB nomenclature.

BI2.1.A - Basic Concept of Enzymes, Isoenzymes & Classify Enzymes

1. Define Enzyme correctly 

1

Lecture

LAQ - Short Notes, SAQ & Viva

2. Define Isoenzyme Correctly

3. Define Co Enzyme & Cofactor Correctly

4. Describe IUBMB system of Classification of Enzyme

BI2.1.B - Coenzymes & Cofactors

1. Describe Classification of co enzyme & state their importance in enzyme catalysis

1

2. Decribe Classification of Co Factor & State their importance in Enzyme Catalysis

3.  Active Centre of Enzymes

BI2.2

Observe the estimation of SGOT & SGPT

BI2.2 A -  Demonstration of SGOT & SGPT  ( BI11.13)

WITH BI11.13

1

P

PRACTICAL

BI2.3

Describe and explain   the basic principles of enzyme activity

BI 2.3 A Mode of Action of Enzymes

1.  Describe Various mechanisms of enzyme activity  

1

Lecture, case discussion

LAQ & Viva, SAQ, MCQ

2. Identiry Different Theory of Enzyme Mechanism of Action

3. Describe various specificities of enzyme action with suitable examples correctly

BI 2.3.B. Enzyme Kinetic

1. Describe Michaelis Constant (km) & Vmax value with suitable diagram

1

2. Enumerate Various factors affecting enzyme activity

BI2.4

Describe and discuss enzyme inhibitors  as poisons and drugs and as therapeutic enzymes

BI.2.4. A - Enzyme Inhbitors

1. Describe Reversible  Enzyme Inhibition with their clinical Importance

1

Lecture

LAQ , Viva, SAQ

2. Describe Irreversible Enzyme Inhibition with Clinical Importance

2. Enumerate Drugs & Poisons which inhibit Enzyme Activity

ECE

3.  Describe Mechanism of Regulation of Enzyme Action

BI2.5

Describe and discuss the clinical utility of various serum enzymes as markers of pathological conditions.

BI.2.5.A - Serum Enzyme as Marker of Pathological Condition

1. Differenciate between Cell Derived & Plasma Derived Enzymes  

1

Lecture, SGT

LAQ, Viva

2. Explain possible Mechanism of Abnormal level of Serum Enzyme in Pathological Condition

3. Enumerate various Enzymes & Isoenzymes and their clinical Importance as Marker of Pathological Condition separately viz. ALT,AST, Amylase, Lipase, LDH, CPK, GGT, 5 nucleotidase, Acid Phosphatase, PSA

2. Describe the enzymes and isoenzymes used as markers of myocardial infarction stating their typical rise-fall with time frame of Actual Incident of MI & interpret them Correctly

Lecture, ECE

Short notes,MCQ,SAQ

3. Describe The enzymes and isoenzymes used as markers of Hepatobilliary Disease & Interpret them correctly

Lecture, ECE

Short Note, MCQ,SAQ

4. Describe The Enzyme and Isoenzyes used as markers for Pancreatic Dieseas & Interpret them Correctly

Lectures, SGT

Short Note, SAQ, MCQ Viva 

5. Describe The Enzyme and Isoenzyme Used as Markers for Bone & Prostate Disease and Interpret them Correctly

Lectures, CCD

SAQ, MCQ, Viva

6. Describe The enzymes and Isoenzymes used as markers for Muscle Diseases & Interpret them correctly

Lectures, CCD

Short Note,MCQ Viva

BI2.6

Discuss use of enzymes in laboratory investigations (Enzyme-based assays)

BI2.6.A. - Enzymes used in Laboratory Investigations

1. Enumerate various assays in which Enzymes used as Diagnostic reagents - For Drugs and chemical Analysis, ElISA, Molecular Diagnosis

1

Lecture

LAQ, Viva

2. Enumerate Therapeutic Use of Various Enzymes

BI2.7

Interpret laboratory results of enzyme activities  & describe the clinical utility of various enzymes as markers of pathological conditions.

Bi2.7.A - Interpretation of Lab results with its clinical utility of various enzymes & Isoenzymes

1. Enlist tissue of Origin of various Enzymes

1

Small Group, Clinical Cases

SAQ, MCQ, Viva

2. Enumerate Biological Reference Range of Various Enzymes of Cardiac, Skeletal Muscle, Hepatobiliary, Pnacrease, Prostate & Bone system

3..advice the relevant enzymatic investigations to be done in the given pathological conditions accurately 

SAQ, MCQ, Viva

4. Interpret the Result of Various Enyzme markers in cardiac, Hepatobiliary, Pancrease, Skeletal Muscle & Bone in Pathological Conditions correctly

5. Explain basis & rationale of Serum Cardiac Markers

Topic: Chemistry and  Metabolism of Carbohydrates

BI3.1

Discuss and differentiate monosaccharides, di-saccharides and polysaccharides giving examples of main carbohydrates as  energy fuel, structural element and storage in the human body

BI 3.1.A  Chemistry of Carbohydrate -monosaccharides & Disaccharides

1. Define , structure,  Functions, classifications and Different protperties of carbohydrates   

1

Lecture, Small group discussion

LAQ, Viva

2.Properties, Reactions and  Biological importance of Monosaccharides 

Lecture, Small group discussion

Short Note, Viva, SAQ

3.Properties and  Biological importance of Disaccharides

Lecture, Small group discussion

Short Note,SAQ Viva

4. Qualitative Tests for Mono & Diasaccharides

1

PRACTICAL

Practical Viva

BI 3.1. B Homopolysaccharides & its impotance 

1. Structure, Sources, Functions & Clinical Significance of Different homopolysaccharides

1

Lecture, Small group discussion

LAQ, Viva

2. Qualitative tests for Polysaccharides

1

PRACTICAL

Practical Viva

BI 3.1. C Heteropolysaccharides & its impotance 

1. Structure, Sources, Functions & Clinical Significance of Different Heteropolysaccharides

1

Lecture, Small group discussion

Short Notes, SAQ & Viva

BI 3.2

Describe the processes involved in storage of carbohydrates and its utilization. 

BI 3.2.A Glycogen Metabolism

1. Process of Glycogenesis with its regulation [Also covered in detail in Competency BI 3.4

1

Lecture,

LAQ, Viva

2. Process of Glycogenolysis with its regulation [Also covered in detail in Competency BI 3.4]   

Lecture

LAQ, Viva

BI3.3

Describe and discuss the digestion and assimilation of carbohydrates from food.

BI 3.3.A Digestion and Absorption of Carbohydrates

1. Mechanism involved in Digestion and Absorption of Carbohydrates with Transporters

1

Lecture

Short Notes, SAQ

2. Various soluble & non-soluble Dietary fibre and its imprtance 

Tutorial

Short Note, Viva, SAQ

3. Enzyme Deficient, Biochemical basis of Clinical Features & Management modalities of lactose intolerance

Lecture

Short Note, Clinical Case

BI 3.4

Define and differentiate the pathways of carbohydrate metabolism, (glycolysis, gluconeogenesis, glycogen metabolism, HMP shunt). 

BI 3.4.A Glycolysis Pathway

1. Aerobic and Anaerobic Glycolysis with its regulation, energy yields and clinical significance of the Pathway

1

Lecture

LAQ, Viva

2. Cori's Cycle and BPG shunt - its clinical significance

Lecture

LAQ, Viva

3.Metabolic fate of  Pyruvate 

Lecture

short Note

BI 3.4.B Gluconeogenesis Pathway

1. Gluconeogenesis pathway with its regulation & clinical significance of the pathway

1

Lecture

LAQ, Viva

2. Malate shuttle

Lecture

Short notes, Viva

BI 3.4. C Glycogen Metabolism

1. Process of Glycogenesis 

1

Lecture

LAQ, Viva

2. Process of Glycogenolysis

Lecture

LAQ, Viva

3. regulation of Glycogen Metabolism and role of cyclic AMP

Lecture

Short Note, SAQ, Viva

4. Glycogen storage disease and their salient features

Lecture

LAQ, CCD,SAQViva

BI 3.4. HMP Shunt pathway

1.Oxidative & nonoxidative phae of Hexose monophospahte pathway

1

Lecture

LAQ,  Viva, SAQ

2. regualtion of the HMP pathway

Lecture

Short Note, Viva

3. physiologic Significance of the pathway- role of NADPH

Lecture

Viva, MCQ

4. Clinical significance of the HMP pathway- clinical manifestation of G6PD deficiency asoociation with malaria

Lecture

Viva, SAQ, Clinical Case 

BI 3.5

Describe and discuss the regulation and integration of carbohydrate metabolism along with associated diseases/ disorders. 

BI 3.5. A integration of carbohydrate metabolism

1. Regulation and integration of different carbohydrate metabolic pathways

1

Lecture

Short Notes, Viva,

2. Different disorders assocaited with carbohydrate metabolism -- G6PD deficiency, Glycogen storage disease, Galactosemia, hereditery Fructose intolerance, Diabetes mellitus

Lecture

CCD, Short Notes, LAQ, MCQ

BI 3.6

Describe and discuss the concept of TCA cycle as an amphibolic pathway and its regulation. 

BI 3.6.A TCA Cycle

1. Steps of citric acid cycle 

1

Lecture

LAQ, Short NOtes, Viva, MCQ

2. Regulation and inhibitors of the TCA cycle

Lecture

3. Significance of citric acid cycle

Lecture

4 Amphibolic & Anaplerotic role of TCA cycle

Lecture

BI 3.7

Describe the common poisons that inhibit crucial enzymes of carbohydrate metabolism (e.g. fluoride, arsenate).  

BI 3.7.A poisons inhibits the pathway 

1. Action of poisons that inhibit the Glycolysis and TCA cycle  

1

Lecture

Short Notes, MCQ

BI 3.8

Discuss and interpret laboratory results of analytes associated with metabolism of carbohydrates. 

BI 3.8.A Different laboratory analytes with carbohydrate metabolism 

1.  Different laboratory Tests done & interpretation of it in G6PD deficiency, Galactosemia, Fructose metabolism 

1

Lecture, Clinical Cases

Clinical Case, Viva

2. various Laboratory tests & their interpretation in Diabetes mellitus

Small Group teaching

Viva

BI 3.9

Discuss the mechanism and significance of blood glucose regulation in health and disease. 

BI 3.9.A  Regulation of Blood Glucose

1. Factors maintaining the normal Plasma Glucose

1

Lecture

LAQ, Short NOtes, VIva

2. Effects of Hormones on maintainance of Glucose Homeostasis - especially Insulin & Glucagon

Lecture

Short Notes, SAQ, MCQ

3. Interpret the results of blood Glucose level (Normal, Hypoglycemic & Hyperglycemic conditions)

Clinical Cases, Small Group Teaching

Case Report

BI 3.9.B Diabetes Mellitus

1. Define & Classify the DM

1

Lecture

SAQ, Viva

2. Metabolic derangements in DM and correlate with clinical manifestation

Lecture

Short Notes, SAQ, Viva

3. Metabolic Syndrome- its diagnostic criteria & clinical sequelae 

Lecture

Short Notes, SAQ, Viva

4. Acute Complications in Diabetes mellitus

Lecture

Short Notes, SAQ, Viva

5. Chronic Complications of Diabetes Mellitus

Lecture

Short Notes, SAQ, Viva

6. Management of Diabetes Mellitus

Lecture

Short Notes, SAQ, Viva

BI 3.10

Interpret the results of blood glucose levels and other laboratory investigations related to disorders of carbohydrate metabolism. 

BI 3.10.A 

1. To Estimate Plasma Glucose concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

1

PRACTICAL

PRactical Viva

2. Oral Glucose Tolerance test - Principle, Pre requisite, Contraindications, Procedure, Interpretation of Normal & Abnormal GTT Tests with Clinical Causes 

Small Group Teaching

Short Notes, SAQ, Viva, Case Report

2. Role of Glycated Hemoglobin in Diagnosis of DM-    

Small Group Teaching

SAQ, Viva

3. Role of Fructosamine , S. Insulin & C Peptide level in Disorders of Cabohdrate Metabolism

Lecture

Viva, MCQ, SAQ

4. Various Laboratory Investigations for Complications related to Diabetes Mellitus

Lecture

Short Notes, MCQ

Number

COMPETENCY
The student should be able to

Learning Objectives (At the end of session, Phase-1 MBBS student shall be able to)

No. of Hours Required

Teaching Learning method

Assessment Type

TOPIC: CHEMISTRY AND METABOLISM OF LIPIDS

BI 4.1

Describe and discuss main classes of lipids (Essential/nonessential fatty acids, cholesterol and hormonal steroids, triglycerides, major phospholipids and sphingolipids) relevant to human system and their major functions

BI 4.1.A Chemistry, Classification & Functions of Lipids

1. Define lipids, Chemistry, Classification, Properties & Function of Lipids

1

LECTURE

SAQ, LAQ, , MCQ

2. Chemistry, Classification, Characteristics, Properties & Biological importance of Saturated, Unsaturated Fatty Acids and their deficiency disorders

3. Biological importance of Polyunsaturated Fatty Acids

BI 4.1.B Triglycerides

1. Structure, Properties & Functions of Simple Lipids-Triglyceride

1

2. Chemistry & biological importance of Cholesterol

BI 4.1.C Phospholipids

1. Classification, Structure & Functions of Different Phospholipids

1

LECTURE

SAQ, LAQ, MCQ, 

2. Mention the action of Phospholipase

3. Role of Pulmonary Surfactant & their Clinical Application

4. Classification,structure & functions of Sphingolipids 

BI 4.2 

Describe the processes involved in digestion and absorption of dietary lipids and also the key features of their metabolism. 

BI 4.2..A Digestion & Absorption of Lipids

1. Digestion and Absorption of Dietary Lipids & their abnormalities

1

LECTURE

LAQ, SAQ

2. Role of Bile Salt in Digestion & Absorption of Dietary lipids

BI4.2.B. Oxidation of Fatty Acids

1. Beta oxidation of Fatty Acids

1

LECTURE

LAQ, SAQ, 

2. oxidation of Odd Chain Fatty Acids & Alpha & Omega Oxidation

BI 4.2.C De novo Synthesis of Fatty Acids

1. De-novo synthesis of Fatty Acids- Steps, Energetics, Regulation & Disorders

1

LECTURE

LAQ, SAQ

BI 4.2.D Adipose tissue Metabolism

1.  Lipid  Metabolism in Adipose tissue 

1

LECTURE, SMALL GROUP TEACHING

LAQ, SAQ, CCD, 

2. Lipid Alteration during well-fed condition, fasting Condition, Diabetes 

3. Metabolic changes in Obesity and Metabolic Syndrome with reference to lipid profile 

4. Fatty Liver & Lipotropic factors

BI 4.2.E Metabolism of Ketone Bodies

1. Ketogenesis & Ketolysis  

1

LECTURE, SMALL GROUP TEACHING

SHORT NOTES, CCD

2. clinical significance of Ketone Bodies in Starvation & Diabetes Mellitus

BI 4.2.F Metabolism of Cholesterol

1. Biosynthesis of Cholesterol & its regulation

1

LECTURE

LAQ, SAQ, VIVA

2. Biological role of Cholesterol & its clinical significance

BI 4.3

Explain the regulation of lipoprotein metabolism & associated disorders. 

BI 4.3.A  Lipoprotien Metabolism

1. Synthesis, Metabolism & Function of Different Lipoproteins

1

LECTURE

LAQ, SAQ, CCD

2. Role of Different Apolipoprotein in Lipoprotein metabolism

3 Hypo And Hyper lipidemia with its management

BI 4.4

Describe the structure and functions of lipoproteins, their inter-relationship amongst different lipoproteins in relation to atherosclerosis. 

BI 4.4.A Lipoprotein Chemistry & Functions

1. Structure, Composition, Characteristics & Functions of Different Lipoproteins with Diagram

1

LECTURE

LAQ, SAQ,, VIVA 

2. Various Methods for Liprotein Separation  

BI 4.4.B Atherosclerosis

1. Role of Lipoproteins in Pathogenesis of Atherosclerosis

1

LECTURE

LAQ, SAQ, MCQ, VIVA

2. Etiology, Metabolic Changes, Risk Factors, Prevention & Management of Atherosclerosis

BI4.5

Interpret laboratory results of analytes associated with metabolism of lipids. 

BI 4.5.A List of Investigations & their clinical significance

1. Interpretation of Lipid Profile Report

1

CCD

CASE REPORT, SAQ, MCQ, VIVA

2. LDL/HDL ratio, Apo-B/Apo A1 

3. Lp (a) 

4. Non HDL Cholesterol

5. Homocysteine, hS -CRP

6. Lipoprotein Associated Phospholipase A2

BI4.6

Describe the therapeutic uses of prostaglandins and inhibitors of eicosanoid synthesis

BI4.6.A  Eicosanoids

1. Define , Classification & Synthesis of Eicosanoids 

1

LECTURE

LAQ, SAQ, VIVA, 

2. Structure, Classification, Biosyntheis, Regulation, biological Actions & Clinical Applications of PGs

3. Inhibitors of Eicosanoids

BI4.7

Interpret laboratory results of analytes associated with metabolism of lipids. 

Same as BI4.5

TOPIC: CHEMISTRY AND METABOLISM OF PROTEIN

BI5.1

Describe and discuss structural organization of proteins. 

BI5.1.A Structure & Classification of Amino Acid

1. Define Amino Acid, Structure

1

LECTURE

SAQ, MCQ, VIVA

2. Classification of Amino Acid based on Structure, Nutritional Requirment  & Metabolic Fate

LECTURE

SAQ, MCQ, VIVA

BI5.1.B Properties & Different Reactions of Amino Acid

1. Isoelectric Point & Ampholite Nature of Amino Acid, Optical Activity of Amino Acids

1

LECTURE

SAQ, MCQ, VIVA

2.  Reactions of Amino acid due to Carboxyl Group, Amino Group & Side Chains 

LECTURE

MCQ, VIVA, 

3. Define Protein & Peptide Bond formation

LECTURE

SAQ, VIVA

4. classify proteins based on various criteria giving suitable examples   

LECTURE

LAQ, SAQ, MCQ, VIVA

BI5.1.C Color Reactions of Amino Acid & Protein

1.Principle & Demonstration of Procedure , Inferences of Various Color reactions & Precipitation Reaction of Proteins

1

DOAP, PRACTICAL

PRACTICAL, VIVA

BI5.1.D Structure of Protein

1. Primary , Secondary , Tertiary & Quarternary Structural Organization of Protein with diagrams and examples along with stabilizing forces 

1

LECTURE

LAQ, SAQ, MCQ, VIVA

2. Brief about study of Protein Structure & with various Methods determining Protein Structure

LECTURE

MCQ, VIVA, 

BI5.2

Describe and discuss functions of proteins and structure-function relationships in relevant areas e.g., hemoglobin and selected hemoglobinopathi es.
(

BI5.2.A Biologically Active Peptides

1. Eneumerate various Biologically Important Peptides, their Structure & Functions & Clinical Significance

1

LECTURE

LAQ, VIVA, MCQ

2. Structure - Function Relationship of proteins as Enzyme, Transport Protein & Structure Protein

LECTURE

SAQ, VIVA

Bi5.2.B Collagen

1. Structure, synthesis & Functions  of Collagen,.

1

LECTURE

LAQ, SAQ, VIVA

2. Degradation & Abnoramalities in Collagen Synthesis & Maturation

LECTURE

LAQ,CASE REPORT

BI.5.2.C Hemoglobin

1. Explain Structure Function & Note on their structural Abnormalities . (REF to BI.6.12)

BI5.3

Describe the digestion and absorption of dietary proteins. 

BI 5.3.A Digestion and Absorption of Amino Acids

1. Principle food stuff in which protein is present

1

LECTURE

VIVA

2. Digestion of Proteins with help of different enzymes & its abnormalities

LECTURE

SAQ, MCQ, VIVA

3. Absorption of Proteins and its abnormality 

LECTURE

SAQ, VIVA

4. Meister Cycle & Clinical Application

LECTURE

LAQ, SAQ, MCQ, VIVA

BI5.4

Describe common disorders associated with protein metabolism

BI 5.4.A Urea Cycle& its disorders

1. Formation of Ammonia with Transamination & Oxidative deamination 

1

LECTURE

LAQ, MCQ, VIVA

2. Detoxification of Ammonia & Urea Cycle with regulation

3. Disorders of Urea Cycle

BI 5.4.B Aromatic Amino acids Metabolism-1

1. Degradation of Phenylalanine & Tyrosine

1

LECTURE

SAQ, MCQ,VIVA

2. important Specialized Products from tyrosine

LECTURE

SAQ,MCQ, VIVA

3. inbron error associated with Phenylalanine

LECTURE

SAQ,MCQ,VIVA, CASE REPROT

BI 5.4. C A Aromatic Amino acids Metabolism-2

1. Catabolism of Tryptophan

1

LECTURE

SAQ, VIVA

2. biologically important substances produced form tryptophan

LECTURE

SAQ, VIVA, MCQ

3. inbron error associated with Tryptophan

LECTURE

SAQ,VIVA,CASE REPORT

4. compounds formed from Histidine & their relevance

LECTURE

SAQ,VIVA

5. Role fo Proline

BI 5.4.D Metabolism of Glycine, Serine & Sulfur containing Amino acids

1. Formation, Utilization, Metabolic Functions & errors related to Glycine

1

LECTURE

SAQ, MCQ, VIVA

2.catabolism and Metabolic functions of Serine

3.Metabolic Functions of sulfur containing Amino acids

BI 5.4. E Metabolism of Branched Chain Amino acids

1. Metabolism & Physiological importance of Branch chain AA & their Inborn errors

1

LECTURE

SAQ,VIVA, LAQ

2. Formation, Biochemical role & therapeutic uses of Nitric Oxide

3. Significance of Polyamines

BI 5.4.F one carbon Metabolism

1. Generation and Utilization of one carbon

1

LECTURE

LAQ,SAQ,VIVA, MCQ

BI5.5

Interpret laboratory results of analytes associated with metabolism of proteins. 

BI 5.5.A Inborn error of Amino Acid Metabolism- prenatal screening & newborn screening

1. Interpret the result of chromatogram for diagnosis of aminoaciduria   

1

LECTURE

SAQ,VIVA

2.  Various lab investigations to diagnose phenylketonuria, alkaptonuria, tyrosinemia, Hartnup's disease, cystinuria, cystinosis, homocystinurias, maple syrup urine disease & interpret it

LECTURE

SAQ,CASE REPORTS, VIVA

TOPIC: METABOLISM AND HOMEOSTASIS

BI 6.1 

Discuss the metabolic processes that take place in specific organs in the body in the fed and fasting states. 

BI 6.1.A Integration of Metabolism

1.  Interrelationships between the metabolic pathways of carbohydrate, lipids & proteins
Metabolic Profile during Well fed stage

1

LECTURE

LAQ,SAQ,VIVA

2. Metabolic adaptation during Fasting and Starvation

3.  organ specialized integration of metabolism (liver, adipose tissue, skeletal muscle, cardiac Muscel & brain) in fed state & fasting state

BI 6.2

Describe and discuss the metabolic processes in which nucleotides are involved. 

BI 6.2.A Overview of Purines & Pyrimidines

1. Purines & Pyrimidine Bases- Structure

1

LECTURE

SAQ, MCQ, VIVA

2. Nucleosides & Nucleotides- Structure, composition & Functions

3. synthetic analogue of Purine & Pyrimidine bases & their derivative serve as anticancer drugs

BI 6.2. B Biologically important Nucleotides

1. Biologically important Nuclotides-Purine & Pyrimidine baases

1

LECTURE

LAQ, SAQ, MCQ, VIVA

BI 6.2.C Synthesis of Purine Nucleotides

1. Denovo Synthesis of Purine Nucleotides

1

LECTURE

LAQ, SAQ, MCQ, VIVA

2. Salvage Pathway for Purine Synthesis

3. Regulation of Purine Synthesis

4. Purine Synthesis Inhibitors

BI 6.2.D. Catabolism of Purine Nucleotides and Disorders

1. Degradation of Purine Nucleotides

1

LECTURE

SAQ, VIVA

BI.6.2.E Pyrimidine Metabolism

1. De novo synthesis of Pyrimidine

1

LECTURE

LAQ, SAQ

2. salvage Pathway of Pyrimidine

3. Degradation of Pyrimidine Nucleotide

4.  Regulation of Pyrimidine Synthesis

BI.6.3

Describe the common disorders associated with nucleotide metabolism. 

BI.6.3.A Disorders of Purine & Pyrimidine Nucleotides

1. Disorders of Purine Nucleotides - Gout

1

LECTURE, SMALL GROUP TEACHING

LAQ, SAQ, ECE  MCQ, VIVA

2. Lesch Nyhan syndrome / Hypouricemia

3. Disorders of Pyrimidine  Metabolism - Orotic Aciduria

BI.6.4

Discuss the laboratory results of analytes associated with gout & Lesch Nyhan syndrome

BI.6.4.Laboratory Investigations and their interpretation in various Disorders of Purine Metabolism (WITH BI6.3)

1. Normal Range & Clinical Importance of Uric Acid

2. Laboratory Investigation in Gout & Lesch Nyhan Syndrome

BI.6.5

Describe the biochemical role of vitamins in the body and explain the manifestations of their deficiency. 

Bi.6.5.A Vitamin A

1. Explain   Vitamin A w.r.t. dietary sources, RDA, Absorption, Transport of Vitamin A

1

Tutorial, Seminar

LAQ, SAQ, CCD  MCQ, VIVA

2 biochemical functions of Vitamin A

3. Wald's Visual Cycle

4. Deficiency Maifestation & toxicity

5. Assessment of Vitamin Deficiency

BI.6.5.B Vitamin D

1. Explain   Vitamin D  w.r.t. dietary sources, RDA

1

Tutorial, Seminar

LAQ, SAQ, CCD  MCQ, VIVA

2 Formation of Vitamin D & Activation of Vitamin D 

3. Biochemical Effects on Different sites like Intestine , Bone & Kidney

4. Deficiency Manifestation - Rickets, Types Clinical Feature, Symptoms , Signs & Management

5. Toxicity of Vitamin D

BI.6.5.C Vitamin E & Vitamin K

1. Explain Vitamin E w.r.t Dietary Sources, RDA, Absorption, Transport

1

Tutorial, Seminar

SAQ,MCQ,VIVA

2. Deficiency Manifestation, Treatment & Toxicity of Vitamin E

3  Explain Vitamin K w.r.t Dietary Sources, RDA, Absorption, Transport

4  Deficiency Manifestation, Treatment & Toxicity of Vitamin K

BI.6.5.D Vitamin B1, B2, B3

1. Explain each Vitamin with their Structure, RDA, Dietary Sources, Biochemical Function- Role as Coenzyme, Deficiency Manifestation & Treatment

1

Tutorial, Seminar

SAQ,CASE REPORTS, VIVA

BI.6.5.E Vitamin B5, B6, B7

1. Explain each Vitamin with their Structure, RDA, Dietary Sources, Biochemical Function- Role as Coenzyme, Deficiency Manifestation & Treatment

1

Tutorial, Seminar

SAQ,CASE REPORTS, VIVA

BI.6.5.F Vitamin B12 & Folic Acid

1. Explain each Vitamin with their Structure, RDA, Dietary Sources, Absorption & Transport ,Biochemical Function- Role as Coenzyme, Deficiency Manifestation, Assessment  & Treatment

1

Tutorial, Seminar

SAQ,CASE REPORTS, VIVA

BI.6.5.G Vitamin C

1. Explain Structure, Metabolism, RDA, Sources, Biochemical Functions, Deficiency Manifestation & Treatment

1

Tutorial, Seminar

SAQ,CASE REPORTS, VIVA

BI.6.6

Describe the biochemical processes involved in generation of energy in cells

BI.6.6.A 

biochemical processes involved in generation of energy in cells

1

Tutorial, Seminar

SAQ, VIVA

BI.6.7

Describe the processes involved in maintenance of normal pH, water & electrolyte balance of body fluids and the derangements associated with these. 

BI 6.7.A Concept of pH and Acid Base Balance

1. Define Acids, Bases, pH & Buffers

1

LECTURE

LAQ, SAQ, MCQ, VIVA

2. Henderson-Hasselbalch's equation & its clinical application

3. Different Buffers of the body fluids

4.Mechanism of regulation of pH (Defence mechanism)

5. Respiratory mechanism for regulation of pH  

6.Renal mechanism for regulation of pH

7.Cellular Buffers

BI 6.7.B Acid-Base Balance & Disorders

1. Define Acidosis, Alkalosis, Compensatory responses & mixed responses

1

LECTURE

LAQ, SAQ, CASE REPORT, MCQ, VIVA

2. Various types of Acid Base disturbances- define, causes, compensatory mechanism, blood investigations & management

BI 6.7.C  Water & electrolyte balance

1. Body Water compartments & their composition 

1

Tutorial,

SAQ, CASE REPORT, VIVA

2. Regulation of Sodium & water Balance

3. Assessment of Fluid & Electrolyte balance

4. Disturbances in Fluid & Electrolyte balance

5. Distribution & regulation of intra & extracellular cations

6. Dehydration & Water Intoxication

BI.6.8

Discuss and interpret results of Arterial Blood Gas (ABG) analysis in various disorders

BI.6.8.A ABG

Clinical Significance, Normal Range of Arterial Blood Gas Parameters, Interpretation of Values & Management of Acid Base Disorders 

1

Tutorial,

ECE

BI.6.9

Describe the functions of various minerals in the body, their metabolism and homeostasis. 

BI.6.9.A. Calcium Homeostasis

1. Dietary Source, RDA & Absorption of Calcium

1

Tutorial, Seminar

LAQ, SAQ, CASE REPORT, MCQ, VIVA

2. Biochemical Functions of Calcium

3. Factor Regulating Blood Calcium Level

4. Hypo & Hypercalcemia

BI.6.9.B. Iron Homeostasis

1. Dietary Source, RDA & Function of Iron

1

Tutorial, Seminar

LAQ, SAQ, CASE REPORT, MCQ, VIVA

2. Absorption, Transport & Storage of Iron in Body

3. Clinical Conditions resulting from Deficiency & Excess of Iron

4. Laboratory Test for evaluation of Iron Status

BI.6.9.C Other Micronutrients

1. Dietary Source, RDA, Biochemical Functions & Related Abnormalities of Various Other Micronutrients

1

Tutorial,

SAQ, MCQ, VIVA

BI.6.10

Enumerate and describe the disorders associated with mineral metabolism

Same as BI.6.9

ECE, CCD

BI.6.11

Describe the functions of haem in the body and describe the processes involved in its metabolism and describe porphyrin metabolism. 

BI.6.11.A Function & Synthesis of Heme and its  Disorder 

1. Haem Functions as BI.5.2

1

LECTURE

LAQ, SAQ, MCQ, VIVA

2. Structure of Haem & Biosynthesis of Heme

3. Regulation of Heme Synthesis

4. Explain Porphyrias ,types of Porphyria, their Enzyme Defect,  Inheritance, Clinical Manifestations & Diagnosis.

BI.6.11.B. Heme Catabolism

1. Describe Catabolism of Heme

1

LECTURE

SAQ,VIVA

BI.6.11.C Heme Catabolism - Jaundice

1. Explain Different Types of Jaundice and their Causes, 

1

LECTURE

LAQ, SAQ, CASE REPORT, MCQ, VIVA

2. Differential Diagnosis of Jaundice by various laboratory tests

BI.6.12

Describe the major types of haemoglobin and its derivatives found in the body and their physiological/ pathological relevance. 

BI6.12.A Hemoglobin

1. Hemoglobin - Structure & Functions

1

SMALL GROUP TEACHING

LAQ, SAQ, MCQ, VIVA

2.  Oxygen Dissociation curve  & Bohr Effect

SMALL GROUP TEACHING

SAQ, VIVA

2. Derivative of Hemoglobin & its clinical Relevant

SMALL GROUP TEACHING

SAQ, MCQ, VIVA

BI6.12.B Hemoglobinopathy

1. Classification of  Hemoglobin Variants

1

SMALL GROUP TEACHING

SAQ, VIVA, MCQ

2. Sickle Cell Disease -  Describe genesis, molecular basis, Pathophysiology, Clinical Features, Laboratory Diagnosis, Management 

SMALL GROUP TEACHING

LAQ, SAQ, MCQ,VIVA, CASE REPROT

3. Thallesemia -Describe genesis, molecular basis, Pathophysiology, Clinical Features, Laboratory Diagnosis, Management 

SMALL GROUP TEACHING

LAQ, SAQ, MCQ,VIVA, CASE REPROT

BI.6.13

Describe the functions of the kidney, liver, thyroid and adrenal glands (WITH BI 6.14 & 6.15)

BI .6.13. A Functions of Kidney

1

Tutorial, Seminar

Short Notes, Viva, MCQ, CCD

BI .6.13. B Functions of Liver

1.  Based on synthetic, metabolic, detoxification, excretory and storage function of liver 

1

Tutorial, Seminar

Short Notes, Viva, MCQ, CCD

2. Describe LFT on basis of Laboratory tests, Estimation method, Normal Range, Interpretation & Clinical Significance

BI .6.13. C Functions of Thyroid

1

Tutorial, Seminar

Short Notes, Viva, MCQ, CCD

BI .6.13. D Functions of Adrenal Glands

BI.6.14

Describe the tests that are commonly done in clinical practice to assess the functions of these organs (kidney, liver, thyroid and adrenal glands). (WITH BI 6.13& 6.15)

BI.6.14.A Renal Function tests

1.  Based on Glomerular function, Tubular function & Complete analysis of physical properties of urine 
2. Clinical significance of biochemical parameters in Glomerular and Tubular diseases 

1

Tutorial

3. Estimation of Kidney Function Tests-Creatinine & Urea 

BI.6.14.B Liver Function tests

1.  Based on synthetic, metabolic, detoxification, excretory and storage function of liver 

1

Tutorial

Short Notes, Viva, MCQ, CCD

2. Estimation of Liver Function Tests- Liver enzymes 

BI.6.14.C Thyroid Function tests

1. Synthesis & Metabolism of thyroid Hormones

1

Tutorial

Short Notes, Viva, MCQ, CCD

2. Estimation of thyroid Hormones

BI.6.14.D Adrenal  Function tests

1. synthesis & Metabolism of Adrenal hormones

1

Tutorial

Short Notes, Viva, MCQ, CCD

2. Test to detect Adrenal Functions

BI.6.15

Describe the abnormalities of kidney, liver, thyroid and adrenal glands (With 6.13 & 6.14)

BI.6.15.A

1. Abnormality of Kidney

1

Tutorial

Short Notes, Viva, MCQ, CCD

BI.6.15 B

2. Abnormality of Liver

1

Tutorial

BI.6.15 C

3. Abnormality of Thyroid

1

Tutorial

Short Notes, Viva, MCQ, CCD

BI.6.15 D

4. Abnormality of Adrenal Glands

1

Tutorial

NO.

COMPETENCY
(The student should be able to)

Learning Objectives  (At the end of session, Phase-1 MBBS student shall be able to)

No. of Hours Required

Teaching Learning method

Assessment Type

TOPIC: MOLECULAR BIOLOGY

BI.7.1

Describe the structure and functions of DNA and RNA and outline the cell cycle. 

BI.7.1.A Structure & Functions of DNA

1.Watson-Crick model of DNA structure with their different properties & functions

1

Lecture

LAQ, SAQ, MCQ,Viva

2. Types of DNA

3 .Higher level of Organization of DNA

BI.7.1.A Structure & Functions of RNA

1. Types of RNA

2. Structure, Functions & Characteristics of Each RNA

BI.7.2

Describe the processes involved in replication & repair of DNA and the transcription & translation mechanisms. 

BI.7.2.A Replication Process

1. Process of Replication, Meselson-Stahl Experiment, Replication Bubble & okazaki segment with polymerase enzymes in Prokaryotes 

1

Lecture

LAQ, SAQ, MCQ,Viva

2. Inhibitors of Replication & their Clinical Significance

3. replication in Eukaryotes

4. Telomerase & Telomere

BI.7.2.B DNA Damage & Repair

1.Types of DNA Damage

1

Lecture

LAQ, SAQ, MCQ,Viva

2. DNA repair Mechanism

BI.7.2.C DNA Transcription 

1. DNA Transcription process in both prokaryotes & Eukaryotes

1

Lecture

LAQ, SAQ, MCQ,Viva

2. Inhibitors of Transcription & their clinical Significance

BI.7.2.D Post Transcription Modification

1. Different Post Transcription Modification with their Clinical Significance

1

Lecture

LAQ, SAQ, MCQ,Viva

BI.7.2.E Genetic Code

1. Genetic Code &their Salient Features

1

BI.7.2.F Translation Process

1. Translation Process in both Prokarotes & Eukaryotes

1

Lecture

LAQ, SAQ, MCQ,Viva

2. inhibitors of Translation eith their Clinical Significance

BI.7.2.G Post translational Modification

1. Post Tanslational Modification with its importance

1

Lecture

LAQ, SAQ, MCQ,Viva

BI.7.3

Describe gene mutations and basic mechanism of regulation of gene expression. 

BI.7.3.A Mutation

1. Define & Classification of Mutation with their sutaible examples

1

Lecture

LAQ, SAQ, MCQ,Viva

2.Effects & Manifestation of mutation

BI.7.3.B Regulation of Gene expression 

1. Concept of gene expression

1

Lecture

LAQ, SAQ, MCQ,Viva

2. various mechanism of regulation of gene expression in prokaryotes and eukaryotes 

3. Lac Operon concepts & its regulation

BI.7.4

Describe applications of molecular technologies like recombinant DNA technology, PCR in the diagnosis and treatment of diseases with genetic basis. 

BI.7.4.A Recombinant DNA Technology

1.Define Recombinant Technology

1

Lecture

MCQ, LAQ, SAQ,Viva

2. Role of Restriction endonuclease

3. Different types of Vector

4. Procedure of DNA recombination

5. Applications of recombinant technologgy

BI.7.4.B PCR Tachnique

1.Principle & Steps of PCR Technique

1

lecture

2. Clinical Application ^ Types of PCR Technique

Gene Therapy

Blotting Technique

RFLP

BI.7.5

Describe the role of xenobiotics in disease

BI.7.5.A Detoxification & Biotransformation of Xenobiotics

1. Define Xenobiotics/ Biotransformation

1

Tutorial, Seminar

SAQ, MCQ, Viva

2. Mechanisms of detoxification

3. Phase I, Phase II and Phase III reactions involved in biotransformation of chemicals and the drugs  

4. importance of Cytochromes in Drug metabolism & Alcohol Metabolism 

BI.7.6

Describe the antioxidant defence systems in the body. 

BI.7.6.A Antioxidants

1. Define & classify Antioxidants

1

Tutorial, Seminar

SAQ, MCQ, Viva

2.Free Radical Scavenger system-Enzymatic & nonenzymatic

3. Preventive & Chain breaking Antioxidants

BI.7.7

Describe the role of oxidative stress in the pathogenesis of conditions such as cancer, complications of diabetes mellitus and atherosclerosis. 

BI.7.7.A Free Radicals 

1. Define free Radicals & generation of ROS

1

Tutorial, Seminar

SAQ, MCQ, Viva

2.Damage produced by ROS & Lipid peroxidation

3. Clinical Significance of Free Radicals

TOPIC: NUTRITION

BI 8.1 

Discuss the importance of various dietary components and explain importance of dietary fibre. 

BI.8.1.Nutrition part -1

1. describe the major components of food and their daily
energy requirement, Sources & Importance

1

Tutorial, Seminar

SAQ,MCQ, Viva

2 enumerate different types, functions and chemical nature of
dietary fibres

3 Nitrogen Balance

4 Various Nutritional Indices

BI 8.2

Describe the types and causes of protein energy malnutrition and its effects. 

BI.8.2.PEM

1. describe PEM and its types w.r.t. clinical features, laboratory
findings and management

Tutorial

LAQ, SAQ, MCQ,Viva

2. Difference between Kwashiorkor & Marasmus

BI 8.3

Provide dietary advice for optimal health in childhood and adult, in disease conditions like diabetes mellitus, coronary artery disease and in pregnancy. 

BI.8.3.A Nutriton in varaious Conditions - Childhood, Pregnancy , Old Age

1. Define Calorie and Mention calorific value of Carbohydrate , Protein & Fat

1

Tutorial, Seminar

LAQ, SAQ, MCQ,Viva

BI.8.3.B Nutrition in various diseases like DM, Heart Disease

2. Calculate  Daily calorie requirement  for  given condition with special reference to RQ, SDI, BMR, BMI & Additional RDA

3. Mention specific Nutritional Crtierial for each given condition

4. Identify Portion of Major and Minor Nutriton in Daily Diet as per Requirement

5. Special Reference in Disease condition for the Supplements required in Diet

BI 8.4

Describe the causes (including dietary habits), effects and health risks associated with being overweight/ obesity. 

BI.8.4.A Obesity

1 Classify Obesity  with Reference to BMI

1

LEcture

SAQ, CCD,VIVA,MCQ

2. Expalin Various Causes leads to Obesity

3. Various  Hormones  & Factors associated with Obesity

4. Effect of Obesity on Health & diseases associated with Obesity

BI 8.5

Summarize the nutritional importance of commonly used items of food including fruits and vegetables (macro-molecules & its importance). 

BI.8.5.A Importance of Macro & Micro Nutrients

1. Summarize the nutrional Importance of various Food Items

1

Tutorial

SAQ, CCD,VIVA,MCQ

TOPIC: EXTRACELLULAR MATRIX

BI 9.1

List the functions and components of the extracellular matrix (ECM). 

BI.9.1.A  ECM

1. Composition of ECM with its constituents     

1

LEcture

SAQ, VIVA

2. Structural and functional properties of constituents of ECM (collagen, laminin, fibrillin and fibronectin) 

3. Glycosaminoglycans and proteoglycans as constituent of ECM   

BI.9.2

Discuss the involvement of ECM components in health and disease.
(

BI.9.2.A Disorders related to ECM

1.  explain in brief the diseases of bone and cartilage 

1

Lecture

SAQ, VIVA, SEMINAR

2. explain in brief the disorders related to collagen, elastin, fibrillin and fibronectin 

3.  enumerate disorders of glycosaminoglycans and proteoglycans affecting integrity of ECM in disease   

BI.9.3

Describe protein targeting & sorting along with its associated disorders. 

BI.9.3.A Protein Trageting & Its Disorder

1. mechanism of protein targeting  & Role of  signal Peptide 

1

Lecture

SAQ,MCQ, VIVA

2. Role of Golgi Appartus in Protein Sorting

3. Importance of Chaperones

4. Role of Ubiquitin & Disposal of Mis folded Protein

5.  Role of transport vesicles in intracellular transport    

6.  Enumerate diseases related to protein targeting and sorting

TOPIC: ONCOGENESIS

BI.10.1

Describe the cancer initiation, promotion oncogenes & oncogene activation. Also focus on p53 & apoptosis.
(

BI.10.1.A Cancer 

1. Etiology, Predisposing Factors & Carciogenic Agent

1

2.  Oncogene & its importance in Genesis of Cancer

3. Role of Oncosuppressor Gene

BI.10.2

Describe various Biochemical Tumor Markers & Biochemical Basis of Cancer Therapy

BI.10.2.A Tumor Markers

1.  enumerate different tumor markers with associated cancers 

1

Tutorial

LAQ, SAQ, MCQ,Viva

2.  applications of tumor markers w.r.t. detection, diagnosis, monitoring , classification/ staging of cancer and monitoring of the treatment 
K 

3. Cancer Chemotherapy & Radiotherapy

4. Prevention of Cancer

BI.10.3

Describe the cellular and humoral components of the immune system & describe the types and structure of antibody.
(

BI.10.3.A Immuno Chemistry

1. Types of Immunity, Its Mechanism & Theories involved in Diversity of Immune Response

1

Lecture

LAQ, SAQ, MCQ,Viva

2. Immunoglobulin Structure & Function

3. Auto Immune Disease

4. Application of Immuno chemical Technique Hybridoma Technique

BI.10.4

Describe & discuss innate and adaptive immune responses, self/ non-selfrecognition and the central role of T-helper cells in immune responses

BI.10.4.A Immune Respone

1. describe the role of specialised chemical mediators, complement cascade and specialised white blood cells in mediation of innate immune system in response to antigen presentation  

1

Lecture

SAQ, MCQ, Viva

2. Role of B & T lymphocyte in Immune Response

3.  explain the basis of immunological memory  

BI.10.5

Describe antigens and concepts involved in vaccine development.

BI.10.5.A Vaccine Development & Antigen

1. Describe Antigen w.r.f to source, type, its effect in Human Body

1

Lecture

SAQ, MCQ, Viva

2. Concept of Vaccine Development

3. Concept of Pre clinical & Clincal trial of Vaccine

No.

COMPETENCY

Learning Objectives                                             (At the end of session, Phase-1 MBBS student shall be able to)

No. of Hours 

Teaching Learning method

Assessment Type

Topic: BIOCHEMICAL LABORATORY TESTS

BI11.1

Describe commonly used laboratory apparatus and equipments, good safe laboratory practice and waste disposal.

BI11.1(A) Laboratory Overview, Equipments  & Safety in Labortory

1. Overview of U.G Biochemistry Laboratory & Service Laboratory

3

Small Group Teaching

short Notes, viva, 

2. To Understand use of various Equipments, their principles and Use in Laboratory

3. Safety rules in Laboratory

BI11.1(B) Labortory Waste & their Disposal

1. Generation of various Laboratory Waste

2. Bio Medical Waste Disposal

BI11.2

Describe the preparation of buffers and estimation of pH.

BI11.2(A) pH & Buffers  (BI11.16 pH meter)

1. Prepartion of Buffers & pH Meter

1

Small Group Teaching

short Notes, Viva

BI11.3

Describe the chemical components of normal urine.

BI11.3 To understand various constitutes of Normal Urine

1. To know Physical properties of Normal Urine - pH, Color, Odor, Transparency, Specific Gravity & chemical components like urea, creatinine, uric acid, ammonia, organic & Inorganic Sulfide, Phosphate.

1

Small Group Teaching

Practical, Viva

2.  To understand Normal levels & abnormal levels of Normal Constitutes in Urine and their clinical Application

Small Group Teaching

Practical, Viva

3. To discuss various abnormal constitutes presence in urine, to identify  test for various anbormal constitutes (Demonstration) and their clinical application 

1

Small Group Teaching

Practical, Viva

BI11.4

Perform urine analysis to estimate and determine  normal and abnormal constituents

BI11.4 (A) To perform Test for Normal & Abnormal Constitutes of Urine

1. To perform tests for Normal Constitutes of Urine under expert observation 

1

DOAP, Practical

PRactical , Viva

2. To perform tests for Various Abnormal Constitutes of Urine under expert observation along with Physical Characteristics and Clinical Applications

DOAP, Practical

PRactical , Viva

BI11.5

Describe screening of urine for inborn errors & describe the use of  paper chromatography

BI11.5(A) To identify various Inborn Error of Metabolism from Urine Sample

1. Eneumerate Inborn Error of Metabolism requiring screening of Urine & their clinical significance

1

Lecture

LAQ, MCQ, Viva

BI11.5(B) Use of Paper Chromatography for Detection of constitutes of Inborn Error of Metabolism

1. Principle of Paper Chromatography

Demonst.

Short Notes, viva

2.Use of Paper Chromatography for Detection of Inborn Error of Metabolism Disorders from Urine Sample

lecture

SAQ, MCQ, Viva

BI11.6

Describe the principles of colorimetry

BI11.6(A) Colorimetry Analysis

1. Define Colorimetry 

1

small group teaching

Short Notes, Practical, Viva

2. Priniciple of Colorimeter and Spectrophotometer & Semi Auto ANalyzer

3. Beer's Law & Lambert's Law

4. Understanding of Absorbance and Transmittance with Example

5. Colorimeter Equipments - its parts & their use in Measurement of Absorbance

6. Details of wavelength used in various analytes estimation 

7. Practical demonstration of colorimetry analytes with their Procedure, Use of Stndard solution, Test Solution, Blank Solution, Calculation with stnd concentration values, Derivation of Result & Interpretation of result

BI11.7

Demonstrate the  estimation of serum creatinine and creatinine clearance

BI11.7 (A) Perform Estimation of S. Creatinine from Given Sample

1. To Estimate S. creatinine concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

2. To Understand & calculate Creatinine Clearance value , its importance in Diagnosis of Kidney functions

Practical

Practical , Viva

BI11.8

Demonstrate estimation of serum proteins, albumin and A:G ratio

BI11.8 (A) Perform Estimation of S. Total Protein from Given Sample

1. To Estimate S. Total Protein concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

BI11.8 (B) Perform Estimation of S. Albumin from Given Sample

1. To Estimate S. Albumin concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

Practical

Practical , Viva

BI 11.8© Calculate A: G Ratio & its clinical Importance

1. Calculate A: G ratio with Total Protein & Albumin values, Mention Normal Range, Interpretation & Clinical Coditions associated with it

BI11.9

Demonstrate the estimation of serum total cholesterol and HDL- cholesterol

BI11.9 (A) Perform Estimation of S. Total Cholesterol from Given Sample

1. To Estimate S. Cholesterol concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

BI11.9 (A) Perform Estimation of S. HDL from Given Sample

1. To Estimate S. HDL concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

Practical

Practical , Viva

BI11.10

Demonstrate the estimation of triglycerides

BI11.10 (A) Perform Estimation of S. Triglyceride from Given Sample

1. To Estimate S. Tirglyceride concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

BI11.11

Demonstrate estimation of calcium and phosphorous

BI11.11 (A) Perform Estimation of S. Calcium & S. Phosphorous from Given Sample

1. To Estimate S. Calcium & S. Phosphorous concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

BI11.12

Demonstrate the estimation of serum bilirubin

BI11.12 (A) Perform Estimation of S. Billirubin from Given Sample

1. To Estimate S. Bilirubin concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

BI11.13

Demonstrate the estimation of SGOT/ SGPT

BI11.13 (A) Perform Estimation of S. GOT  & SGPT from Given Sample

1. To Estimate S. GOT & SGPT concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

BI11.14

Demonstrate the estimation of alkaline phosphatase

BI11.14 (A) Perform Estimation of S. ALP from Given Sample

1. To Estimate S. ALP concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

BI11.15

Describe & discuss the Composition of CSF.

BI11.15(A) CSF Physical & Chemical Characteristics with Abnormal findings & its Clinical Correlations

1. Discuss about Cerebro Spinal Fluid  - Formation, Circulation, Physical Properties, Chemical Compositon & its normal values

1

Demonstration

SAQ, Viva, MCQ, Clinical Case

2. Demonstraton of various biochemical tests for detection of Compositions of CSF like Glucose, Protein, Chloride, Blood 

3. Clinical Correlation & CSF Pictures seen in various Pathological Conditions

BI 11.16

Observe use of
commonly used
equipment/
techniques in
biochemistry
laboratory
including:

BI11.16 (B)Paper chromatography of
amino acid, Protein electrophoresis
TLC, PAGE

1. Detail understanding of Techniques, Principle, Use in various Diagnostic & therapeutic conditions, Advantage & disadvantage of Techniques AND Demonstration of Technique 

1

small Group Teaching, Demon

Short Notes, Viva

BI11.16©   Electrolyte analysis by ISE
ABG analyzer

1. Detail understanding of Techniques, Principle, Use in various Diagnostic & therapeutic conditions, Advantage & disadvantage of Techniques AND Demonstration of Technique 

1

small Group Teaching, Demon

Short Notes, Viva

BI11.16(D)ELISA
Immunodiffusion

1. Detail understanding of Techniques, Principle, Use in various Diagnostic & therapeutic conditions, Advantage & disadvantage of Techniques AND Demonstration of Technique 

1

small Group Teaching, Demon

Short Notes, Viva

BI11.16(E)Autoanalyser, DNA isolation
from blood/ tissue.

1. Detail understanding of Techniques, Principle, Use in various Diagnostic & therapeutic conditions, Advantage & disadvantage of Techniques 

1

small Group Teaching, Demon

Short Notes, Viva

Quality control

1. Explain QC, its use in Clinical Laboratory, Importance, Violation rules

1

small Group Teaching, 

Short Notes, Viva

2. Difference between Control & calibrator

BI 11.17

Explain the basis
and rationale of
biochemical tests
done in the
following
conditions:

BI11.17 (A) Approach to clinical condition like Diabetes
mellitus,

(1) explain the basis and rationale of plasma glucose, urine glucose, urine ketone bodies, HBA1c, Serum Insulin, Serum Cpeptide
Assay and other special investigations done in Diabetes Mellitus

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.17(B) Approach to clinical condition like Renal Failure, 

(1) explain the basis and rationale of serum creatinine, blood urea, urine creatinine, creatinine clearance and other clearance
tests, urine microalbumin, urine microalbumin/ creatinine ratio,
serum electrolytes done in Renal Failure

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.17(C ) Approach to clinical condition like Dyslipidemia, Myocardial Infarction

(1) explain the basis and rationale of serum cholesterol, serum triglycerides, serum HDL cholesterol, serum LDL cholesterol,
LDL/HDL ratio, Serum Apo-lipoproteins and other special investigations in done in Dyslipidemia

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.17 (D) Approach to clinical condition like Liver Disease, Jaundice

(1) explain the basis and rationale of serum total bilirubin, serum direct bilirubin, serum AST, serum ALT, serum ALP, serum
total protein, serum albumin, urine bile salts and urine bile pigments done to investigate Jaundice

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.17 (E ) Approach to clinical condition like Thyroid Disorder

(1) explain the basis and rationale of serum total T3 and total T4, serum free T3 and T4, serum TSH, serum anti-thyroid
antibodies done to evaluate Thyroid Disorders

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.17 (F) Approach to clinical condition like Acid Base disorder

(1) explain the basis and rationale of ABG Analysis i.e. blood pH, PO2, SPO2, PCO2, serum HCO3-, serum Na, serum K, serum Cl
and anion gap analysis done to investigate acidosis and alkalosis

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.17 (G) Approach to clinical condition like Gout

(1) explain the basis and rationale of serum uric acid and synovial fluid biochemical analysis and microscopy done in
Gout.

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.17 (H) Approach to clinical condition like Myocardial Infaction

(1) explain the basis and rationale of serum cardiac markers viz. serum CPK-MB, serum cardiac troponins, serum LDH-1/LDH-2
ratio, serum AST/ALT ratio, serum myoglobin and other special investigations such as complete lipid profile done in Myocardial
Infarction

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.17 ( I) Approach to clinical condition like Nephrotic Syndrome

explain the basis and rationale of serum total protein, serum albumin, urine microalbumin, urine creatinine, urine microalbumin/ creatinine ratio done to evaluate Proteinuria

1

LCD,
Practical,
Lab Reports,
CCD, ECE

SAQ, Viva, MCQ, Clinical Case

BI11.18

Discuss  the principles of spectrophotometry.

BI1.18 (A) Spectrophotometry

1. Explain principle, Methodology, Indication, Advantage, Disadvantage & uses of spectrophotometer

1

small Group Teaching, demon

SAQ, Viva

BI11.19

Outline the basic principles involved in the functioning of instruments commonly used in a biochemistry laboratory and their applications.

BI11.19 (A) Principle and uses of various equipments used in Biochemistry Laboratory

1. Principle & uses of semi Auto, Fully Auto Analyzers

2. Principle & uses of Autoclave

1

small Group Teaching, demon

SAQ, Viva

3. Principle & Uses of Incubator

4. Principle & uses of Centrifuge

5. Principle & uses of Incubator

6. Principle & Uses of Autopipettes

BI11.20

Identify abnormal constituents in urine, interpret the findings and correlate these with pathological states.

BI11.20 (A) Abnormal constitutes present in urine with its pathological conditions

BI11.3 & BI11.4

Practical

PRactical , Viva

BI11.21

Demonstrate estimation of Glucose, Creatinine, Urea & Total Protein in Serum

With BI11.7 & 11.8

1. To Estimate Plasma Glucose concentration under expert observation & with given test protocol - Principle, Method to be used, Proceudre of Estimation, Caculation of Result, Normal range, Interpretation & clinical conditons associated with it.

2

Practical

Practical , Viva

BI11.22

Calculate Albumin:Globulin Ratio and Creatinine Clearance

With BI11.7 & 11.8

BI11.23

Caclulate Energy content of different food items, Identify food items with high and low glycemic index and explain the importance of these in diet

With BI3.1

BI11.24

Enumerate advantages and/or disadvantages of use of unsaturated, saturated and trans fats in Food

BI11.24 A Fatty Acids types and Advantage & Disadvantage

1) Saturated , Unsaturated & Trans Fatty acids - Food Items containing these, Their Advantage & Disadvantages

Tutorial

MCQ,SAQ, Viva